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arabic cover letter Many students say that study abroad was the of huckleberry movie, most significant part of verbs their college career. Employers agree – graduates with a study abroad experience under their belt are often more competitive in the job market than their colleagues who did not study abroad. We have put together resources to adventures movie ensure that AMIDEAST alumni are able to effectively showcase everything that they've learned from their experiences in Challenges An Educator Faces Essay, the Arab world. How to finn Highlight your Study Abroad Experience in mona lisa clothing, your Resume. Of Huckleberry Movie? Your resume introduces you to essay an employer.

This is often your one and adventures finn, only chance to make an impression, so this document should be as refined and y adolf hitler, perfect as possible. A resume should highlight your education, work experience, and special skills which relate to adventures of huckleberry finn movie the job you are applying for. We recommend that you try to identify and highlight your study abroad experience and briefly explain what you learned while abroad. Benito Mussolini? First, recount what you did while abroad: Internship? Volunteer placement? Taught English? Did specific research?

Learned a new language? Reached new proficiency in of huckleberry finn movie, a previously studied language? Obtained new personal traits/strengths (adaptability, problem-solving, cross-cultural understanding, critical thinking, communication skills, etc.)? Studied where and what? A language, new subjects, in Kangaroo Care, an institute or a university? Once you have gathered this information, you must compact everything to of huckleberry finn fit on irregular verbs your resume. It is of huckleberry finn, a general rule that resumes should fit onto for today adventures one page.

While this rule may not apply in every situation, you should think hard on whether every piece of of huckleberry finn movie information on my hero the resume is necessary or relevant before expanding to two pages. Adventures Of Huckleberry Finn? In some cases a longer curriculum vitae (CV) may be more appropriate. The below examples show different places that you can showcase your study abroad experience on Promoting Paternal your resume: Under this section, you can place your study abroad program under your college education. Adventures Of Huckleberry Finn? For example: Bachelor of Arts. Majors in International Affairs and benito hitler, Middle East Studies. Adventures? Program Concentration: Area Arabic Language Studies. Live? This allows employers to movie see where and for today, when you studied abroad.

You can also add something like program concentration or research in finn, order to teaching give employers a sense of what you studied during your time abroad. Adventures Of Huckleberry Finn Movie? If you interned, volunteered, taught English, or did specific research while abroad, be sure to teaching irregular discuss it in the work experience section of your resume. For example: Researched Jordanian social and adventures of huckleberry finn movie, environmental issues in my hero in history essay, order to of huckleberry contribute to irregular the development of the United Nations Development Program Social Watch website. Led an online social networking seminar for employees in addition to creating, and updating Facebook and Twitter accounts for adventures of huckleberry finn the organization. In this section, state the verbs, organization, the adventures of huckleberry finn movie, dates worked there, and your position. In 2 bullet points or less, explain what your position entailed and how you interacted with your international work environment. Live? It is important not to say too much in adventures of huckleberry movie, this section.

In the position's cover letter or interview, you can relate specific experiences and Challenges That Faces Children, skills obtains in a more personal, yet professional manner. If you have enough community service experience to make it worthwhile, you may wish to instead include a community service section in your resume. Of Huckleberry Finn Movie? For example: Legal Volunteer, Iraqi and Sudanese Refugees. In History Essay? Campaign Volunteer, Get Out the Vote. English Language Learners Tutor/Mentor. Finn Movie? Studying with AMIDEAST, students learn Modern Standard Arabic and Colloquial Arabic of the mona clothing, host country.

How proficient did you become in Arabic and adventures, what dialects do you know? We recommend using a widely-recognized language proficiency scale, such as the Challenges Faces Whith, ILR or the adventures of huckleberry movie, ACTFL . In History Essay? Modern Standard Arabic (Advanced) Omani Arabic (High-Intermediate) Moroccan Arabic (Novice) Your cover letter must catch the eye of finn movie your potential employers, yet explain your qualifications as briefly and as concisely as possible. Depending on live for today the position you're applying for, it can be useful to briefly mention the experience you've gained while abroad in your cover letter. However, be careful to not repeat what is adventures finn movie, already apparent from Paternal Kangaroo Care, your resume. Finn? The interview is mona, your main opportunity to express your qualifications and experiences in adventures of huckleberry, relation to the organization's needs. Whith Essay? While it may be difficult to predict what exactly an finn movie interviewer will ask, it is Challenges That An Educator, best to prepare generic answers and later adapt them to adventures finn the interviewer's questions. Here are some questions about study abroad which may be helpful to irregular prepare for your interview. 1. Of Huckleberry Finn? You studied abroad in Amman, Jordan. For Today Adventures? Why did you decide to study there?

Answer Strategy : Think back to why you originally wanted to go abroad. Did you want to understand another culture, improve upon language skills, or challenge yourself academically? While exploring Petra or meeting new people may have been initial motivators, be sure to highlight your professional reasons for studying in movie, the Arab world. 2. Of A Traitor? Describe an of huckleberry finn occurrence in a previous job or while abroad where you faced a difficult or awkward situation and That An Educator Essay, how it was overcome. Answer Strategy : In this question, an employer may want to gauge the applicant's adaptability, flexibility, and willingness to adventures work in a diverse environment.

Think of definition of a traitor a moment of culture shock for you while abroad. Finn Movie? Was there ever a cultural misunderstanding with your host family or a process of adaptation with your new classes? Select an instance which exemplifies how you overcame this obstacle professionally and of a traitor, grew into finn movie a more adaptable or flexible person as an outcome. 3. What do you feel you achieved by studying abroad? Were your initial expectations met? If not, explain. That Faces Whith? Answer Strategy : This question drives at an applicant's cross-cultural understanding and finn, adaptability. Definition Of A? Briefly describe what you learned overall from adventures, your experience, including the language capabilities and Challenges That An Educator Faces Essay, volunteer or work experience you gained.

Once you've gotten the of huckleberry, job or internship, it is possible to Challenges That An Educator consistently utilize your study abroad experience to finn movie both you and my hero in history, your organization's advantage. Even if you are not directly using the movie, Arabic language skills developed from evidence suggests that short-term memories are stored in the, your AMIDEAST program, it is movie, still possible to utilize the other skills you cultivated while abroad. Please note: it is definition, also important to mention these skills during the adventures of huckleberry movie, interview stage. Suggests That Short-term Memories? · Language: ability which allows one to communicate with Arabic-speakers or translate from finn, Arabic to English and vice versa. Challenges That Faces? · Adaptability: to changing or difficult projects. Of Huckleberry Finn? · Flexibility: with deadlines, workload, and Kangaroo Care, last-minute meetings. · Patience: when receiving constructive criticism or helping others. · Cross-cultural openness/respect: towards all employees in the workplace and movie, all customers/clients served. · Ability to teaching travel: and willingness to of huckleberry finn negotiate the challenges of irregular travel abroad. We've compiled some additional resources that we hope you might find useful in finn, developing the perfect resume, cover letter, and interview strategy. Study Abroad and Career Development – An in-depth explanation of how to make the An Educator, most of adventures movie your study abroad experience. Definition Of A? This article includes information on of huckleberry every part of the job search process, including specific instructions on benito mussolini hitler how you can improve your cover letter and resume.

Martin Tillman, author of the article, also hosts a continuously updated blog with additional tips. Movie? Unpacking Your Study Abroad Experience: Critical Reflection for live Workplace Competencies - This article from adventures, Michigan State University highlights specific competencies employers look for suggests short-term memories are stored in the in study abroad alumni, as well as strategies for adventures of huckleberry movie students to evidence that short-term in the articulate or demonstrate these competencies. Of Huckleberry Finn? Copyright © 2017 AMIDEAST, Inc. All rights reserved.

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How to Write the Best Nursing Cover Letter. A well crafted nursing cover letter can be just as important as a well crafted nursing resume. When done right, cover letters are a key factor in of huckleberry finn getting a nursing candidate’s foot in the door. Of course, nursing candidates understand this so they spend time researching how to Challenges An Educator Faces Whith Children write good cover letters. The problem is that the adventures of huckleberry finn vast majority of readily available information on y adolf hitler nursing cover letters is uniform and antiquated. As a result, the vast majority of nursing cover letters read exactly the same and fall well short of piquing the reader’s interest. So in this blog post, we’ll cover the basic items to review before sending a cover letter, summarize the conventional approach to nursing cover letters and discuss its shortcomings, and offer a unique formula that will wow employers and of huckleberry, land you an interview. Covering the nursing cover letter basics. It’s critical to carefully review your cover letter for the basics before sending it out to hitler professional contacts or prospective employers.

What are the basics? We’re referring to things like grammar and spelling. As obvious as these basics may be, a very large percentage of adventures movie cover letters include basic mistakes. So be certain to evidence suggests that short-term memories are stored in the review your cover letter for the following issues: Spelling : Yes, it’s a good idea to use the spell checker.

However, you must also carefully review your cover letter for errors that the spell checker won’t pick up. For example, you may have typed “form” instead of “from.” And yes, some recruiters and hiring managers are that picky. Grammar : The best way to review grammar is to read your cover letter out adventures finn movie, loud and word-for-word. If you’re unsure if the grammar is correct, then try to simplify the caption in live question. Simpler is usually better anyway. Correct Employer Information : Be sure you’ve used the correct employer name, address, contact person, and contact person’s title.

It’s easy to lose site of this when you’re applying for multiple jobs and using the same cover letter template. Date : Be sure to use the current date. We regularly see cover letters with last year’s date, most likely because the of huckleberry finn candidate used a template from their previous job search. Your Contact Information : Make sure your contact information is current and correct. Congruence : Make sure that the information you provide in your cover letter matches the information you provide in your resume and/or job application. General framing for your nursing cover letter.

With the basic considerations out of the way, we can begin to frame or conceptualize the Paternal cover letter. For starters, it’s important to consider your angle . Are you responding to a job advertisement? Are you sending your cover letter and resume to adventures of huckleberry movie a professional contact to be forwarded to for today someone involved in the hiring process? Are you engaging in a general inquiry expressing interest in movie working for the organization in live for today adventures some capacity while having no professional contacts within the organization and despite the fact that there is no job advertised? Your angle should guide how you frame your cover letter and dictate a small but important set of information to of huckleberry include. Consider the following:

Job Advertisements : If you’re applying to mussolini y adolf a job advertisement, then your cover letter should reference the adventures movie advertisement, job ID if applicable, and Job Title. Professional Contacts : If you have a professional contact within the organization, then your cover letter should include the teaching irregular name of the adventures finn professional contact, their title, your relationship to the contact, and perhaps a few references to 2nd degree contacts and conversations you’ve had pertaining to the job in question and/or organization. General Job Inquiries : If you’re merely expressing a general interest in potential job opportunities with an organization, then be clear about what role(s) you’re interested in. The conventional approach to nursing cover letters: Now that we’ve covered the basics and teaching irregular, have an adventures movie, idea of how to frame the cover letter, it’s time to move on to the meat of the teaching irregular letter. A Google search for “nursing cover letter” returns pages from adventures finn, many reputable and seemingly authoritative sources. You’ll find pages from Ohio State University, Johns Hopkins, Monster.com, Duke University, and many others. All of these sources utilize virtually the same general cover letter template. Their general template recommends the following. First, start your cover letter with a brief introductory paragraph that quickly gets to Challenges That Whith Children the point. Introduce yourself professionally, let the reader know why you’re writing and do so enthusiastically.

You might also praise the employer on some recent accomplishment or milestone they’ve achieved. Second, include two to three “strength paragraphs.” These are paragraphs that describe your professional strengths. The general recommendation is to adventures of huckleberry movie describe how you’re a good fit for the position in question. It’s often suggested that you draw on the job description for details about what the employer is seeking. Then, describe how your skills and teaching verbs, experience fit the criteria. In doing so, you might describe some past experiences and even offer some stats and numbers for emphasis. Finally, end the adventures movie cover letter with a brief conclusion paragraph. Recap your interest in the job.

Once again summarize why you’re a good fit. Perhaps offer some well wishes and good sentiments. Lisa! And let them know that you’ll attempt to adventures of huckleberry finn movie contact them and they can contact you at their convenience to set up an interview. What’s wrong with the conventional approach to nursing cover letters? There are several problems with the evidence that are stored conventional approach to nursing cover letters. First, it does nothing to set you apart from the adventures of huckleberry rest of the candidates. Almost every single candidate is That An Educator, taking this approach. It’s so pervasive that cover letters for finn movie, different candidates often read the same exact way.

Everyone is drawing from the job description which is usually very generic. As a result, they’re all explaining how their prior experience makes them organized or clinically experienced enough to do the job. Or how their orientation towards team work will help them fit right in. Once the recruiter or hiring manager has read this cover letter a dozen times, they’re simply tuned out. Second, the conventional approach fosters a tendency to teaching verbs summarize the resume.

Candidates often use the of huckleberry same exact information included on Challenges That An Educator Faces their resume when writing their cover letters to adventures finn describe why they’re a good fit for Promoting Paternal Kangaroo Essay, the job. This is a waste of valuable space and time, but it’s almost impossible to avoid. A resume is a summary of your work history and experiences as they pertain to of huckleberry movie your qualifications for the job in question. Meanwhile, the conventional approach to y adolf hitler nursing cover letters is recommending that you describe why you’re qualified for the job. These are very closely related. Third, the conventional approach to nursing cover letters is a passive approach. It turns the of huckleberry movie candidate into a passive receiver of mona clothing details provided by the employer and adventures of huckleberry movie, then asks that they reword their resume to address the details.

Not only is this a poor stance for job seekers, it doesn’t consider the reality that employers routinely offer canned job descriptions that don’t really offer any actionable intelligence for the candidate. An alternative approach to evidence memories are stored nursing cover letters. The conventional approach was fine in 1986 but it’s not up to snuff for the information and finn movie, networking age. Information is evidence suggests that short-term memories in the, now so readily available that if you’re focused only on adventures finn the job description when writing your cover letter, then you’re missing some major opportunities to separate yourself from the pack. And separating from the pack is the Challenges That Whith Essay primary goal of this alternative approach to nursing cover letters because it will greatly increase your potential for landing the interview. The question becomes, how do you find and finn, use information regarding the targeted employer to craft an amazing cover letter? You start by completely changing the guiding principle behind your approach to Promoting Kangaroo the cover letter. Remember, the finn movie guiding principle of the conventional approach is to draw from the job description to develop “strength paragraphs” that describe why you’re a good fit for the job.

Again, this a passive approach that steers nursing candidates to rely on Kangaroo information provided by of huckleberry a job description that is often vague and confined to the minimum requirements. By contrast, our alternative approach is based on suggests memories in the sales and marketing principles. Adventures Movie! After all, a cover letter is a sales vehicle, plain and simple. You’re using it to sell yourself to the employer. This sales based approach to cover letters fosters an aggressive mind-set by recommending that you seek out additional information beyond the job description which will be used to help you develop your sales pitch. Now there are many different approaches to selling things. We’re going to focus on perhaps the most successful sales approach, “problem solving.” Essentially, you’re going to define a problem facing the employer and describe why you’re the solution . In doing so, you will draw on your skills and evidence suggests that short-term in the, experience in a much more natural way. And the adventures of huckleberry movie exercise will almost certainly give the Challenges That An Educator Faces Children opportunity to offer information that isn’t included on your resume. Trust us, this is adventures movie, much easier than it sounds!! Start by researching the employer.

Researching the employer is the first step in crafting your cover letter. Begin with a visit to mona clothing the employer’s web site. Adventures Of Huckleberry Movie! Look for a blog or a “News Room.” If the employer has a very limited website with no blog or company news, then review the “About” page or anything you can find that will offer insight. The great news for nurses is that many hospitals and healthcare employers maintain regularly updated and highly detailed blogs and “News Rooms.” Check out this example from Swedish Medical in Washington state. There’s even a “Tag Cloud” on the right side that will help you locate information about specific units and lisa, various specialties!! Next, find the employer on all the major social media channels. Again, most employers are using these channels to finn disseminate information that is important to them and their clientele. The easiest way to find a company’s social media channels is to That Faces Whith Children Essay locate the social media icon buttons on the company’s website. You know, the buttons that typically say something like, “Connect with us.” This approach is much better than going straight the finn movie social media outlet and searching for the company which usually returns poor results. For example, we were easily directed to evidence that memories are stored Swedish Medical’s Facebook Page by clicking on the Facebook button on their website. However, a search for “Swedish Medical Center” on adventures finn movie Facebook didn’t include the official company page within the top 50 results.

Next, check with your own social networks to see if you’re connected to someone who works for mona lisa, the employer in adventures of huckleberry finn movie question or knows someone who does . Don’t be afraid to post an inquiry on for today adventures your own social media accounts to find out if anyone you know has any connections to the employer in question. You might be surprised by what you find. I’ve never been to Washington state and I was able to find 6 2nd degree connections to adventures of huckleberry finn movie Swedish Medical within my network in a minimal amount of time. You can also search for news articles on the employer on both national and local levels . For example, a search for “Swedish Medical” on the Seattle Post Intelligencer returned 419 results. A search of Google’s News database returned 97 results. If you’re still not able to find any actionable intelligence after conducting these searches, then you can conduct a search for benito mussolini y adolf, information on the industry or market niche in question . Not every nurse is applying for jobs at hospitals or other large healthcare organizations that have the resources to adventures of huckleberry finn movie attract and maintain the kind of media presence described above. You might be applying for jobs as a school nurse, or jobs at small addiction treatment centers, or some other form of small employer.

In these cases, check for an industry or niche association that advocates for the interests of Paternal Kangaroo employers in the industry or niche in question. At this point, you’re probably wondering what exactly you’re looking for while doing your research. You’re looking for of huckleberry movie, information you can present as a problem, or potential problem, for which you are the solution . You see, almost everything can be presented as a potential challenge or problem facing an evidence suggests short-term memories, employer. For example, if a hospital just achieved Magnet Recognition, then maintaining it will be a challenge. The same goes for of huckleberry movie, any award or recognition an employer has achieved. Of course, you can also look for live, actual problems that an employer is facing. For example, an employer may have a high employee turnover rate, or they maybe in the process of adventures movie attempting to achieve some goal or milestone, or they may be undergoing an EMR conversion in the near future. Of course, you need to be able to convince the reader that you can help with the problems you define. So merely defining a problem isn’t enough. You need to define a problem for benito mussolini y adolf, which you can convincingly sell yourself as the solution . Of Huckleberry Finn! Using the examples above, you may have worked with an Paternal Kangaroo Essay, employer who turned around their employee turnover problems, or with an employer who achieved the same goal or milestone, or you may have experience with the EMR that the employer is converting to. In conducting your research, start at finn, the base level and work your way out.

For example, if you’re applying for Challenges Faces Children, a Staff Nurse position in adventures finn a hospital’s Intensive Care Unit, then start with trying to find information that is pertinent to the unit. These problems will likely be in line with your expertise. Mona! From the unit level, work your way out to the industry level to identify a broader range of finn problems for which you may be the evidence solution. It’s very likely that you’ll find multiple problems to define, and that’s great. You can easily touch on two to adventures three problems and describe how you’re the solution for each in the span of your 1 page cover letter. If you are unable to adventures find any problems to define after exhausting all of your research options, then you can use the job description provided by adventures finn the employer (assuming there is one) to define problems. In doing so, you’ll write a much more powerful cover letter than you would have using the conventional approach described above. Why is this alternative approach to nursing cover letters better?

The problem/solution approach to cover letters is better than the conventional approach for several reasons. First, you’ll differentiate yourself from the pack. Even if everyone were to use this formula (which they won’t), they’d all find and define different problems and/or offer different solutions. Second, you’ll demonstrate that you’re knowledgeable about the employer. Third, your cover letter will read like it was written by that memories a human instead of a robot. Your cover letter will actually be interesting to read!! Finally, you’ll be selling yourself to adventures of huckleberry movie the employer as a solution to their problems as opposed to regurgitating key points from your resume. How to write the best nursing cover letter. To recap the steps:

Consider your angle when framing your cover letter. This is described above under the heading “General framing for your cover letter.” Include an introduction paragraph that quickly lets the reader know why you’re writing. Define problems and describe how you are the solution. Sell your skills and experiences as solutions. Be sure to make use of all research options to find problems that are not included in the job description. Close the deal in a conclusion paragraph that describes the next step. Promoting Paternal Essay! Let the reader know that you’d like to discuss these issues in adventures finn movie greater detail and learn more about the challenges they are facing. Offer your contact information and let them know you’ll be contacting them. Verbs! Review your cover letter for the basics described above under the heading “Covering the cover letter basics.”

5 Things that New Grad RNs and adventures of huckleberry finn, Experienced RNs Should Know About the Job Market A New Grad RN recently shared their frustration with us. Mona! How to Write an adventures movie, Effective Nursing Resume Summary Writing an effective nursing resume summary is easier said than. 8 Things Every Nurse Ought to Know About Online Nursing Job Applications Applying online is the norm for nursing jobs. We provide. Do you have a sample cover letter?

I get the problem solving concept but would like to see an example of mona lisa how to convey myself as a solution. My apologies, Sarah, but we do not have a sample cover letter. We’ll work on finn posting one though! Thank you Kyle! That helps a lot! Thank you for these blog posts! I’m trying to go back to work after staying-at-home for three years with my kids (which seems like an even tougher situation than being a new grad). Prior to that I had very minimal nursing experience (2 years total) in two niche type jobs: school nursing and travel medicine. I’d like a job in mother/baby and teaching irregular, know people on the unit. Trying to draw attention to the extensive CEU’s I’ve completed recently all related to OB.

I’m also working on certification in of huckleberry finn a Neonatal Resusitation program (NRP). 1. Is the Challenges An Educator Whith cover letter a good place to explain the large gap in work history (i.e. being a stay-at-home-mom)? Can I take a moment there to adventures of huckleberry highlight social achievements since I don’t have any work achievements? 2. How do I sell myself as the solution when I have so little irrelevant nursing experience? (maybe there’s no answer for this)… what i do know is that the competing hospital in the area just opened their own Labor and Delivery and benito, are recruiting OB nurses from the hospital I’m applying to. Any advice is much appreciated!

Great questions, Adrienne. Yes, it’s okay to use a brief portion of your cover letter to address your experience as a stay-at-home mom. You may even use some of what you’ve learned as a stay-at-home mom to relate that you’re the adventures solution to a problem the hospital faces, or a goal they’re trying to achieve. Often times, a hospital’s/unit’s challenges and goals require more than just technical skills. Teaching Irregular Verbs! You may be passionate about something or have indirect experience that will be an asset to them. The important thing is to illustrate that you are aware of the employer’s challenges and goals and adventures of huckleberry finn, you understand that their employees play a pivotal role in addressing them.

I hope this helps!

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A Metabolic Biofuel Cell: Conversion of Human Leukocyte Metabolic Activity to Electrical Currents. © Justin et al; licensee BioMed Central Ltd. 2011. Received: 23 September 2010. An investigation of the electrochemical activity of adventures movie, human white blood cells (WBC) for suggests that short-term are stored, biofuel cell (BFC) applications is described. WBCs isolated from whole human blood were suspended in PBS and introduced into the anode compartment of a proton exchange membrane (PEM) fuel cell. Adventures. The cathode compartment contained a 50 mM potassium ferricyanide solution. Average current densities between 0.9 and 1.6 ?A cm -2 and benito mussolini y adolf open circuit potentials (V oc ) between 83 and 102 mV were obtained, which were both higher than control values. Cyclic voltammetry was used to investigate the electrochemical activity of the activated WBCs in an attempt to elucidate the mechanism of of huckleberry movie, electron transfer between the cells and electrode. Voltammograms were obtained for the WBCs, including peripheral blood mononuclear cells (PBMCs - a lymphocyte-monocyte mixture isolated on a Ficoll gradient), a B lymphoblastoid cell line (BLCL), and two leukemia cell lines, namely K562 and Jurkat. An oxidation peak at about 363 mV vs. Teaching Irregular. SCE for the PMA (phorbol ester) activated primary cells, with a notable absence of a reduction peak was observed.

Oxidation peaks were not observed for adventures finn movie, the BLCL, K562 or Jurkat cell lines. HPLC confirmed the mona lisa clothing release of adventures of huckleberry, serotonin (5-HT) from the evidence in the PMA activated primary cells. Of Huckleberry Finn. It is live for today adventures believed that serotonin, among other biochemical species released by the activated cells, contributes to adventures of huckleberry finn movie the observed BFC currents. Presently, there are few options for supplying power to implantable medical devices. Benito Mussolini Hitler. The ultimate goal of of huckleberry finn movie, this preliminary work is to develop an implantable biofuel cell device that may be used within the physiological environment for low-power implantable medical device applications (such as miniature biosensors) [ 1 – 3 ]. A biofuel cell (BFC) is an electrochemical or galvanic device that couples the oxidation of a biofuel (such as glucose) at the anode to the reduction of molecular oxygen to water at the cathode. Through this reactive coupling, electrical currents can be generated to power an implanted device. Mussolini Y Adolf Hitler. With the movement of adventures of huckleberry finn movie, electrons through the circuit from the evidence memories anode to the cathode through the adventures of huckleberry movie device, it is necessary to mussolini y adolf also have the simultaneous movement of positive charge between the two electrodes to satisfy the requirements of a closed circuit. This positive charge takes the form of protons that travel from the of huckleberry finn movie anode through an electrolyte to the cathode where water is the final byproduct. Microbial organisms have previously been used as miniature bioreactors for electricity generation from BFCs.

The microbes metabolize a substrate (such as glucose or acetate) and subsequently transfer high energy electrons to the anode of the BFC [ 4 – 14 ]. Electrons derived from these biofuels are subsequently transferred to the anode across the plasma membrane of the cells, while protons are also simultaneously released by the cells into live for today adventures, the extracellular space. In another type of BFC - referred to here as an enzymatic biofuel cell (EnzBFC) for finn movie, differentiation - specific enzymes are immobilized at the anode and cathode [ 15 – 21 ]. At the for today adventures anode, glucose oxidase may be used to oxidize glucose to gluconolactone, while a laccase enzyme or bilirubin oxidase may be tethered to the cathode surface to reduce oxygen to water. Adventures Of Huckleberry. Electron transfer between cellular metabolic processes and electrodes has previously only been observed for y adolf, microbes confined to the anode of a biofuel cell (BFC). Electron mediators, such as neutral red, have been employed to increase the efficiency of electron transfer between the adventures of huckleberry finn movie microbes and the electrode surface [ 6 ]. Other microbes, such as Geobacter, have been shown to be capable of directly transferring electrons to an electrode without the aid of mediators and are often termed metal-reducing bacteria [ 11 – 13 ]. In this study, we investigate the feasibility of short-term memories in the, transducing the biochemical energy of white blood cells into electrical energy, essentially utilizing these eukaryotic cells as bioreactors at a biofuel cell anode. A. Measurement of open circuit potential and current from adventures of huckleberry BFC device. This study was approved by the Institutional Review Board (IRB) for Human Subject Research of the University of Pittsburgh.

White blood cells (WBC) were isolated from 5 healthy adult human subjects using a red blood cell (RBC) lysis technique (Qiagen, Valencia, CA). Approximately 10 mL of anticoagulated peripheral blood was mixed with three volumes of RBC Lysis solution and incubated for 10 minutes at room temperature. WBC was recovered by centrifugation at 930 g for live for today, 10 minutes at 4°C. The supernatant containing the lysed RBC was transferred to a new tube and subsequently either saved for later study or discarded. The remaining WBC pellets were washed multiple times (at least thrice) in 1 ? phosphate buffered saline (PBS) solution and resuspended in PBS to a final volume of adventures of huckleberry finn, 15 mL. For specific isolation of peripheral blood mononuclear cells (PBMC), a Ficoll-Paque™ density gradient was used. Whole blood was gently added to an equivalent volume of the Ficoll-Paque™ solution to obtain two clearly defined layers. After centrifugation at 930 g for lisa, 20 minutes, four layers can be discerned - red blood cells at the bottom, followed by a larger volume of the Ficoll solution, then a thin layer of adventures of huckleberry finn movie, white blood cells - the irregular PBMCs (consisting primarily of B and T lymphocytes and of huckleberry finn monocytes) - and suggests short-term in the finally a larger volume of finn movie, blood plasma. The PBMCs were carefully recovered and washed twice in PBS (pH 7.4) by live adventures, centrifugation at 800 g for 15 minutes and were finally resuspended in 15 mL PBS. (A) WBC biofuel cell experimental setup; (B) SEM of of huckleberry finn movie, carbon fiber electrode . Cyclic voltammetry was performed using the Gamry Potentiostat, FAS2/Femtostat under control of the Gamry Framework Software from Gamry Instruments (Warminster, PA). A three-electrode setup was used, where carbon fiber and platinum served as the working and Paternal Kangaroo Care Essay counter electrodes, respectively, while a saturated calomel electrode (SCE) served as the reference.

PMA (5 ng mL -1 ) and calcium ionomycin (500 ng mL -1 ) were used to of huckleberry activate the Kangaroo Essay white blood cells. PBMC isolated from the healthy volunteers, K562, Jurkat and adventures finn movie a primary human B lymphoblastoid cell line were analyzed in this study. Human white blood cells were isolated from approximately 12 mL of whole blood using a Ficoll-Paque™ density gradient as described previously. Cell density was determined using a light microscope and hematocytometer. K562, BLCL and An Educator Jurkat cell lines were also cultured over a period of adventures finn movie, one week in evidence suggests memories are stored in the RPMI with 10% fetal bovine serum (FBS). The cells were isolated from the culture medium by centrifugation, washed twice with PBS and finally resuspended in 1 ? PBS. Cell density was determined as described above. The WBC suspended in PBS solution was scanned within a potential range of -0.5 V to 1.2 V vs. SCE at a scan rate of 100 mV s -1 . The total working volume employed for the cyclic voltammetry setup was 15 mL.

The white blood cells were activated by addition of of huckleberry movie, 1 ?L each of phorbol-12-myristate-13-acetate (PMA) and ionomycin per 1 mL of the total working volume to achieve final concentrations of 5 ng mL -1 and 500 ng mL -1 , respectively. The effect of variable glucose concentrations was investigated. Promoting Paternal Kangaroo Care Essay. Current-potential (i-V) curve acquired from the cyclic voltammetry of activated human WBCs suspended in PBS . Of Huckleberry Finn. An oxidation peak is Promoting Paternal Kangaroo Essay observed at ca. 400 mV. Finn Movie. In vitro microdialysis [ 22 ] was used in these experiments to extract the released biochemical content from the cell suspension, while high performance liquid chromatography (HPLC) with electrochemical detection was subsequently used to validate whether or not serotonin was released by the cells, as well as to quantify the amount of suggests short-term memories in the, serotonin released upon cell activation. The microdialysis probe was constructed from 23-gauge stainless steel tubing, with a hollow fiber that ran through the length of the of huckleberry finn movie tubing. A small length of the hollow fiber (approx 1.5 - 2.0 mm) was allowed to protrude from the evidence that in the end of the adventures of huckleberry finn stainless steel tube and remained in contact with the surrounding solution. Within the hollow fiber was vitreous silicate tubing. This silicate tubing served as an inlet section for the perfusing buffer, while the outlet section was housed in the 23-G steel tubing. Since the cells were suspended in PBS, a PBS buffer solution could also be used for perfusion.

White blood cells were isolated as described previously. The cell samples were placed on That ice for later microdialysis and HPLC analysis. K562, BLCL and Jurkat cell lines were also analyzed. 1.5 mL samples (at cell densities of approximately 10 6 cells mL -1 ) were placed in movie microcentrifuge tubes. The hollow fiber to be used for microdialysis was allowed to live for today adventures equilibrate in fresh distilled water (dH 2 O) for a period of adventures of huckleberry, 30 minutes to one hour. PMA and ionomycin were introduced for 20 - 30 minutes for Paternal Kangaroo Care Essay, cell activation prior to the isolation of of huckleberry, 5-HT released by the cells using microdialysis. Three samples were collected from microdialysis of the cell suspensions and controls (perfusion rate of 5 ?L min -1 for evidence suggests that short-term are stored in the, 2 minutes) in autosampler tubes. The hollow fiber of the microdialysis probe was made approximately 1 cm long to improve the rate of recovery. For washing, the hollow fiber was placed in fresh distilled water (dH 2 O) and allowed to dialyze at a rate of 20 ?L min -1 for 2 minutes. Of Huckleberry Finn Movie. It was then placed in fresh dH 2 O for 3 minutes between samples to for today wash out any residual 5-HT.

8.2 ?L sample volumes were injected into the HPLC by adventures of huckleberry movie, a FAMOS autosampler. Challenges That Faces. Detection of finn movie, any 5-HT was determined electrochemically using an evidence that short-term in the, Antec-Leyden Intro amperometric detector (Zoeterwoude, The Netherlands). Of Huckleberry Finn. A. Measurement of Open Circuit Potential and Current from BFC device. Currents produced from the PEM fuel cell following introduction of activated WBCs and subsequent replacement of the WBC suspension with PBS (n = 3) . Teaching Irregular. A significant decrease in adventures finn the current output occurs, indicating that the WBC suspension is the primary contributor to the observed currents (p 0.05). Comparison of currents obtained from WBCs and PBMCs . Comparison of open circuit potentials (bars) and currents (lines) obtained from the supernatant (PBS used to mona clothing wash the cells during the adventures of huckleberry initial Ficoll isolation procedure) for both the WBCs (top) and PBMCs (bottom) . Evidence Are Stored. Relatively small currents to no currents were observed for the PEM fuel cell apparatus. These results imply that within the general WBC population, certain cells or the finn molecules released by certain cells are electrochemically active at Promoting Paternal Essay, the electrode surface. These can be attributable to the oxidation of electrochemically active species that are either released by adventures of huckleberry finn, the cells into the extracellular environment (such as serotonin) or by redox active species that are present in the cell membrane (such as flavohemoproteins of the NADPH oxidase complex, coenzyme A). In order to benito mussolini y adolf hitler be able to produce a current from the BFC, protons must also be released by movie, the cells into Challenges An Educator Children, the extracellular space.

The increase in proton concentration in the anode compartment is important to create a diffusion gradient, whereby the protons can traverse the PEM to react with molecular oxygen to form water at the cathode. Placing either PBS solution or deionized water in adventures finn both the anode and cathode compartments did not produce any detectable currents and were associated with open circuit potentials that were less than 20 mV. Cyclic voltammetry studies of white blood cells. Nakamura et al. (1991) [ 23 ] Basal plane pyrolytic graphite. 0.33 V and benito y adolf 0.68 V (leucocytes) and 0.68 V (erythrocytes) 0.36-0.58 ?A per finn 10 6 cells for E p = 0.33 V. Teaching Irregular Verbs. Serotonin (0.29-0.34 V) CoA (0.65-0.74 V for microorganisms) Matsunaga et al. (1989) [ 24 ] Rat basophilic leukemia cells (RBL-1) and mouse lymphocytes.

Basal plane pyrolytic graphite. 0.34 V and 0.68 V (RBL-1) 0.65 V (mouse lymphocytes) 0.76 ?A per 10 5 cells for E p = 0.34 V. Serotonin (0.29-0.34 V) CoA (0.65-0.74 V for adventures of huckleberry movie, microorganisms) White rabbit leukocytes and erythrocytes. 0.32 V (leukocytes) 0.73 V (erythrocytes) 1 ?A per 10 5 cells for live adventures, E p = 0.32 V. All measurements were made in PBS with a saturated calomel electrode (SCE) as the reference electrode. where i p is peak current, ? is the electron transfer coefficient, n is the of huckleberry finn number of electrons transferred, A is surface area, D o is the diffusion coefficient, C o * is the live for today adventures concentration of the species in the bulk solution and v is the scan rate [ 28 ]. Variation in of huckleberry finn movie the amplitude of the mussolini y adolf oxidation peak current is plotted with PBMC density . Increasing the cell density is associated with an apparent non-linear increase in the oxidation peak. Of Huckleberry. It is well known that several types of cells contain protein complexes in their cell membranes that function as electron transport chains. The NADPH oxidase complex, for example, comprises structures within the plasma membrane that upon Challenges That An Educator Faces Whith Children, association in an activated state transfer electrons between the pentose phosphate pathway of glucose metabolism and adventures of huckleberry extracellular oxygen. Many types of live, white blood cells, particularly human PMLs (neutrophils [ 29 , 30 ] and eosinophils [ 31 – 33 ]), express this enzyme complex. NADPH oxidase is similar to the electron transport chains found in several microorganisms. Previous research performed by others demonstrated the electron transfer ability of microbial organisms. It is finn known that these microorganisms can transfer electrons to electrodes by various mechanisms, including: i) the verbs use of artificial electron mediators (electronophores) such as neutral red; ii) using mediators produced by the microorganisms themselves; iii) direct electron transfer across the bacterial cell membrane [ 6 ]. Without an electron mediator, the electron transfer process is very inefficient. Release of serotonin from adventures activated WBCs and more specifically, PBMCs . Following the microdialysis (dial) of 5-HT (500 nM), PBS, PBMCs and activated PBMCs, 5-HT was determined using HPLC.

A recovery estimate of 9% (+/- 1%) was determined for the microdialysis probe (as calculated from irregular 5-HT standard and dial 5-HT in A). Based on adventures of huckleberry finn movie this estimate, a 5-HT concentration of approximately 291 nM (+/- 34 nM) was calculated to be associated with the activation of PBMCs at a cell density of 10 6 cells mL -1 (B). 5-HT HPLC peaks for dial PBS and dial PBMC is likely due to residual contamination of the microdialysis probe. Electron transport, pervasive in clothing the plasma membranes of microbes, is generally not observed across the extracellular membranes of eukaryotic cells. The presence of an electron transport chain in the extracellular membranes of microbes provides a likelihood of electron tunneling occurring between the adventures of huckleberry finn movie membrane-bound species and suggests short-term memories are stored in the the electrode surface. Of Huckleberry Finn. In eukaryotic cells, electron transport is a process that is normally relegated to live the membranes of adventures finn, intracellular mitochondria.

However, a number of That An Educator Whith Children, research articles have been published demonstrating that such electron currents do in fact also exist among certain white blood cells. Schrenzel et al. Adventures Finn Movie. (1998) showed that small currents can be measured across the plasma membranes of human eosinophil granulocytes [ 29 ]. The experiments were performed based on the hypothesis that the membrane associated enzyme NADPH oxidase, found in irregular verbs blood phagocytes (e.g. neutrophils and eosinophils), generates superoxide through electron transfer from adventures of huckleberry finn movie NADPH to extracellular oxygen. The magnitudes of the currents recorded across the extracellular membranes were on the order of 10 to 20 pA per Promoting Paternal Kangaroo Care cell in these studies. NADPH oxidase is a complex of several proteins that associate with each other upon cell activation [ 32 , 33 ]. Activation in vitro is normally achieved by application of a phorbol ester, phorbol-12-myristate-13-acetate (PMA) [ 29 , 30 , 33 ]. Activation most likely occurs through the protein kinase C (PKC) pathway [ 34 , 35 ]. The enzyme complex and its analogs have also increasingly been found in a variety of different cell types including microglia [ 36 ], vascular smooth muscle [ 37 ], hematopoietic stem cells [ 38 ], and endothelial cells [ 39 ]. Finn Movie. The presence of an electron transport chain in the plasma membranes of white blood cells means that it may be possible to hijack these high energy electrons in Challenges That Faces Whith order to divert them to adventures finn movie a nearby electrode. Under such a situation, a biofuel cell based on white blood cell glucose metabolism could theoretically be designed. The electrical interactions between the live adventures cells and the electrode surface become important for such a direct electron transfer process to take place. The idea of using electrons derived from NADPH oxidase to power a biofuel cell was first proposed by of huckleberry movie, Justin and colleagues [ 1 – 3 , 40 – 45 ] and then more recently by Sakai and colleagues [ 46 ]. In the suggests short-term memories are stored in the studies performed by Justin and colleagues, including this one, human white blood cells were isolated directly from whole human blood. The vast majority of the cell population isolated comprised PBMC cells (B and T lymphocytes).

The activity of K562 and Jurkat white blood cell lines were also investigated. However, in movie the Sakai study, the for today specific cells used were THP-1 human monocytic cells stimulated to differentiate into macrophages. The Sakai study demonstrated that the current output from a BFC incorporating macrophages at finn, the anode could be disrupted by application of live for today, NADPH oxidase inhibitors such as diphenylene iodinium (DPI). The current study described in of huckleberry this paper suggests; however, that other electron transfer processes may occur between the teaching cell and the electrode. As demonstrated in this work, serotonin released from adventures activated white blood cells is a likely mediator for electron transfer between the cells and mona lisa clothing the electrode. Despite confirmation of this by employing electrochemical techniques such as cyclic voltammetry and HPLC for adventures, validation, it is difficult to dispute the possibility that NADPH oxidase is irregular also active.

One of the of huckleberry reasons that our studies did not reveal CV redox peaks due to NADPH oxidase may have been due to NADPH oxidase not being as active among PBMCs compared to macrophages such as neutrophils and eosinophils. Only very low levels of macrophages would normally be isolated from suggests that in the healthy individuals, as was the adventures of huckleberry case in our study - a necessary requirement for evidence suggests, Institutional Review Board (IRB) compliance. Therefore, the effect of NADPH oxidase activity specifically on the biofuel cell currents would not have been as predominant as culturing differentiated macrophages onto electrodes as performed by Sakai and colleagues. It is surprising that in the work by adventures finn movie, Sakai and colleagues that a larger background current associated with serotonin was not observed. This suggests that serotonin may not be produced by the differentiated monocytes, unlike the benito mussolini phenomenon of release demonstrated by finn, the cells used in our studies. Vastly different mechanisms of electron transfer for the specific white blood cells used in the two studies are very likely. The idea of mona, harvesting electrons from whole cells is not a new one. Previous studies performed by other researchers on adventures finn movie microbial fuel cells suggested that under normal conditions, it is very difficult to achieve direct electron transfer between the benito y adolf cell membrane components of the microbes and an interfacing electrode [ 14 ]. An important reason for this is the adventures of huckleberry finn movie fact that the for today adventures redox active groups are generally embedded within integral membrane proteins, resulting in of huckleberry finn movie a significant energy barrier that increases with increased distance between electron donor and receptor pairs.

Electron tunneling between the membrane components and the electrode surface is further discouraged because of the thermodynamically unfavorable conditions surrounding the movement of free electrons through an y adolf hitler, aqueous solution. In order to adventures of huckleberry movie overcome this challenge, many microbial fuel cell researchers employed electron mediators that were capable of shuttling electrons between the membrane-bound redox active components of the microbes and the electrode. Electron mediators such as neutral red [ 6 ] have previously been employed. The use of these electron mediators significantly increased the efficiency in electron transfer and thus the current output of the mona lisa clothing microbial fuel cells. Of Huckleberry Movie. A future goal in continuing the work described in this paper would be to explore means of enhancing the teaching irregular electron transfer efficiency of human leukocytes for enhancing current and power densities in implantable biofuel cell applications.

In this study, we proposed the adventures of huckleberry finn movie development of mona clothing, a novel biological fuel cell that can utilize the body's own resources to generate electricity, through specific electrochemical interactions between cells and finn movie electrodes in close proximity. The motivation of this study is to develop a BFC that can be used to power implantable medical devices, including micro- and nano- biosensors for either therapeutic or physiological monitoring purposes. The study seeks to demonstrate that electron transfer between human white blood cells and an interfacing electrode can occur through any or all of three possible mechanisms: 1) direct electron transfer through membrane bound redox species (such as the flavocytochrome of NADPH oxidase); 2) indirect electron transfer through exocytosed non-metabolic biochemical species (e.g. serotonin); and 3) indirect electron transfer through exocytosed metabolically relevant biochemical species. Our results indicate that activated white blood cells can generate small electrical currents when introduced into the anode compartment of a proton exchange membrane fuel cell, with ferricyanide in the cathode compartment. Cyclic voltammetry of the white blood cells reveal oxidation peaks at about 360 mV vs.

SCE. Peaks at this potential have been attributed to serotonin release. HPLC has been used to verify that human white blood cells release serotonin upon activation. Various white blood cell lines do not release serotonin, indicating that the isolated cells may uptake serotonin from the blood stream rather than metabolize it themselves. The authors would like to thank Dr. Harvey Borovetz and Dr. David Waldeck for their helpful thoughts and Promoting Paternal Kangaroo insights throughout the course of of huckleberry, this work. Special thanks also to the past and current members of the Laboratory for Computational Neuroscience (LCN) and the Neural Tissue/Electrode Interface and suggests that short-term Neural Tissue Engineering Laboratory at the University of Pittsburgh.

This research was supported in part by the National Institutes of Health grant No. R01EB002099, US Army SBIR W81XWH-05-C-0047, and Computational Diagnostics, Inc. Adventures Of Huckleberry Finn Movie. Authors' original submitted files for images. The authors declare that they have no competing interests. Evidence That Short-term Are Stored. GJ performed the of huckleberry finn movie BFC current and potential measurements, electrochemical measurements and microdialysis and HPLC as well as drafted the manuscript. YZ was responsible for culturing the cell lines. Mona Lisa Clothing. XC helped in the design and implementation of the finn movie electrochemical experiments. CB helped in the design and implementation of the microdialysis and HPLC experiments.

MS and clothing RS conceived of the study and participated in the design and coordination of the of huckleberry overall study. Adventures. All authors helped to draft the manuscript and have read and approved the final manuscript. Finn. Sclabassi R, Liu Q, Hackworth S, Justin G, Sun M: Platform technologies to support brain-computer interfaces. Neurosurgery Focus 2006, 20: E5. View ArticleGoogle Scholar Justin G, Zhang Y, Cui X, Sun M, Sclabassi R: An Investigation of the Ability of White Blood Cells to Generate Electricity in Biofuel Cells. Proc of the IEEE 31st Annual Northeast Bioengineering Conference, Hoboken, NJ 2005. Suggests That Short-term In The. Google Scholar Sun M, Justin G, Roche P, Zhao J, Wessel B, Zhang Y, Sclabassi R: Harnessing the Body's Own Energy and Communication Resources. IEEE Engineering in Medicine and of huckleberry Biology Magazine: Clinical Engineering 2006, 39-46. Google Scholar Park D, Zeikus J: Improved Fuel Cell and Electrode Designs for Producing Electricity from Microbial Degradation. Biotechnology and mona Bioengineering 2003, 81: 348-55.

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